33-047

PI 3-Kinase 3-step HTRF^® Assay

The PI 3-Kinase 3-Step HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format.

• Synonym(s): 3-Step HTRF Assay, PI 3-Kinase Assay
• UNSPSC Code: 12161503
• NACRES: NA.84
• eCl@ss: 32161000

Properties

• Quality Segment: 100
• species reactivity: human, mouse
• manufacturer/tradename: HTRF^®, Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_006218.2
• UniProt accession no.: P42336
• detection method: fluorometric (HTRF)
• shipped in: dry ice
• Gene Information: human ... PIK3CA(5290)

Description

General description

Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA) gene is mapped to human chromosome 3q26.32. This gene codes for the catalytic subunit p110α of class IA PI3-kinase. PI3K signaling pathways regulate various cellular activities and properties including proliferation, survival, apoptosis, motility, and morphology. Mutation or overexpression of the PIK3CA gene is implicated in the development of various human solid tumors including head and neck cancers or pharyngeal cancer and ovarian cancer.

Application

PI 3-Kinase 3-step HTRF^® Assay has been used to determine the in vitro inhibitory potencies of phosphatidylinositol 3-kinase (PI3K) δ inhibitors such as MSD-496486311, MSD126796721, idelalisib, and duvelisib across PI3K α, β, γ, and δ isoforms.

Biochem/physiol Actions

Target Sub-Family: PI3K/PI4K

Packaging

Kit capacity: 10,000 wells

Preparation Note

12 months at -70°C

Other Notes

4X Reaction Buffer

PIP2 (substrate, 1mM stock)

DM A Reaction Buffer

DM B Reaction Buffer

DM C Reaction Buffer

BPIP3

Stop Reagent

Research Category
Apoptosis & Cancer
Research Sub Category
Oncology

Legal Information

HTRF is a registered trademark of Cis Bio International

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable