07-551

Anti-G9a Antibody

Upstate^®, from rabbit

• Synonym(s): KMT1C
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 50

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_006709
• UniProt accession no.: Q96KQ7
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... EHMT2(10919)

Description

General description

140 kDa

Protein G9a (also known as Histone H3-K9 methyltransferase 3, Euchromatic histone-lysine N-methyltransferase 2 and HLA-B-associated transcript 8)

Immunogen

Peptide (C-DERVDSDSKSEVEALTEQ) corresponding to amino acids 71-88 of human G9a.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-G9a Antibody (Rabbit Polyclonal Antibody) validated in WB to detect G9a also known as KMT1C.

Biochem/physiol Actions

Recognizes human G9a protein.

Physical form

Format: Purified

Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Protein A purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Control
Positive Control Included: HeLa Nuclear Extract (12-309).

routinely evaluated by immunoblot HeLa nuclear extract cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2