05-615

Anti-SUV39H1 Histone Methyltransferase antibody

UPSTATE^®, mouse monoclonal, MG44

• Synonym(s): KMT1A
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: MG44, monoclonal
• Species reactivity: mouse, human
• Application: ChIP, IP, WB
• Citations: 34

Properties

• Product Name: Anti-SUV39H1 Antibody, clone MG44, ascites fluid, clone MG44, Upstate^®
• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: MG44, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_003173.2
• UniProt accession no.: O43463
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SUV39H1(6839)

Description

General description

50 kDa

Post -translational modification of histones, such as phosphorylation, acetylation, and methylation, are crucial for chromatin remodeling. SUV39H1 and Suv39h1 are the human and mouse homologs of Drosophila Su(var)3-9 protein, respectively. These proteins selectively methylate histone H3 at lysine 9 creating a high-affinity binding site for the HP1 proteins (heterochromatin protein 1). HP1 proteins are known to interact with transcription suppressor proteins, suggesting that the interactions with SUV39H1 and methylated histone H3 mediate a silence effect on the transcription of target genes. Furthermore, over-expression of SUV39H1 in HeLa cells causes a redistribution of endogenous HP1 proteins and growth retardation, suggesting that SUV39H1-mediated modulation of heterochromatin can impair cell cycle progression.

Immunogen

MBP fusion protein corresponding to residues 1-412 of human SUV39H1

Application

Anti-SUV39H1 Antibody, clone MG44 is a Mouse Monoclonal Antibody for detection of SUV39H1 also known as KMT1A, Histone H3-K9 Methyltransferase 1 & has been validated in ChIP, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

Biochem/physiol Actions

Recognizes SUV39H1.

Physical form

Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Unpurified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
U2OS cell lysate

routinely evaluated by immunoblot on U2OS cells lysed in RIPA with 0.1% SDS and sonicated

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABE552

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1