06-1304
Anti-SETD8 (hPR-SET7) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Histone-lysine N-methyltransferase SETD8
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SETD8(387893)
General description
N-lysine methyltransferase KMT5A (UniProt: Q9NQR1; also known as EC: 2.1.1.43, H4-K20-HMTase KMT5A, Histone-lysine N-methyltransferase KMT5A, Lysine N-methyltransferase 5A, Lysine-specific methylase 5A, PR/SET domain-containing protein 07, PR-Set7, PR/SET07, SET domain-containing protein 8) is encoded by the KMT5A (also known as PRSET7, SET07, SET8, SETD8) gene (Gene ID: 387893) in human. SETD8 (hPR-SET7) is a histone methyltransferase that adds a single methyl group to lysine 20 of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. SETD8 is not detected during the G1 phase. It is first detected during S through G2 phases, and peaks during mitosis. It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis and is also involved in chromosome condensation and proper cytokinesis. It is also reported to mediate mono-methylation of p53/TP53 at lysine 382, leading to repress p53/TP53-target genes. SETD8 has a SET domain in the amino acids 257-378 and this region contains the active site of enzymatic activity. Sequences upstream and downstream of SET domain are also required for its methyltransferase activity. Two isoforms of SETD8 have been described that are produced by alternative splicing.
Specificity
This rabbit polyclonal antibody detects N-lysine methyltransferase KMT5A (PR-SET7).
Immunogen
GST-tagged recombinant protein corresponding to human SETD8 (hPR-SET7).
Application
Anti-SETD8 (hPR-SET7) Antibody is a Rabbit Polyclonal Antibody for detection of SETD8 (hPR-SET7) also known as Histone-lysine N-methyltransferase SETD8 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
Evaluated by Western Blotting in HeLa cell nuclear extract.
Western Blotting Analysis: 0.05 µg/mL of this antibody detected SETD8 (hPR-SET7) in HeLa cell nucleaar extract.
Western Blotting Analysis: 0.05 µg/mL of this antibody detected SETD8 (hPR-SET7) in HeLa cell nucleaar extract.
Target description
~43 kDa
Linkage
Replaces: 07-316
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
Storage and Stability
Stable at 2-8°C for 1 year from date of receipt.
Analysis Note
Control
HeLa nuclear extract.
HeLa nuclear extract.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Aja Aravamudhan et al.
Physiological reports, 12(7), e15999-e15999 (2024-04-13)
Pulmonary arterial hypertension (PAH) causes pulmonary vascular remodeling, increasing pulmonary vascular resistance (PVR) and leading to right heart failure and death. Matrix stiffening early in the disease promotes remodeling in pulmonary artery smooth muscle cells (PASMCs), contributing to PAH pathogenesis.
Mai Nagasaka et al.
Scientific reports, 13(1), 22877-22877 (2023-12-22)
Transforming growth factor β (TGF-β) is a multifunctional cytokine that induces a diverse set of cellular processes principally through Smad-dependent transcription. Transcriptional responses induced by Smads are tightly regulated by Smad cofactors and histone modifications; however, the underlying mechanisms have
Chieh-Tien Shih et al.
Aging cell, 16(4), 797-813 (2017-05-18)
Cellular senescence is a permanent proliferative arrest triggered by genome instability or aberrant growth stresses, acting as a protective or even tumor-suppressive mechanism. While several key aspects of gene regulation have been known to program this cessation of cell growth
Stine Jørgensen et al.
The Journal of cell biology, 192(1), 43-54 (2011-01-12)
The eukaryotic cell cycle is regulated by multiple ubiquitin-mediated events, such as the timely destruction of cyclins and replication licensing factors. The histone H4 methyltransferase SET8 (Pr-Set7) is required for chromosome compaction in mitosis and for maintenance of genome integrity.
Neysha Tirado-Class et al.
Life science alliance, 7(1) (2023-11-09)
DDB1- and CUL4-associated factors (DCAFs) CDT2 and DCAF14 are substrate receptors for Cullin4-RING E3 ubiquitin ligase (CRL4) complexes. CDT2 is responsible for PCNA-coupled proteolysis of substrates CDT1, p21, and SET8 during S-phase of cell cycle. DCAF14 functions at stalled replication
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