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  • Characterization of Hormone-Dependent Pathways in Six Human Prostate-Cancer Cell Lines: A Gene-Expression Study.

Characterization of Hormone-Dependent Pathways in Six Human Prostate-Cancer Cell Lines: A Gene-Expression Study.

Genes (2020-10-11)
Andras Franko, Lucia Berti, Alke Guirguis, Jörg Hennenlotter, Robert Wagner, Marcus O Scharpf, Martin Hrabĕ de Angelis, Katharina Wißmiller, Heiko Lickert, Arnulf Stenzl, Andreas L Birkenfeld, Andreas Peter, Hans-Ulrich Häring, Stefan Z Lutz, Martin Heni
ANOTACE

Prostate cancer (PCa), the most incident cancer in men, is tightly regulated by endocrine signals. A number of different PCa cell lines are commonly used for in vitro experiments, but these are of diverse origin, and have very different cell-proliferation rates and hormone-response capacities. By analyzing the gene-expression pattern of main hormone pathways, we systematically compared six PCa cell lines and parental primary cells. We compared these cell lines (i) with each other and (ii) with PCa tissue samples from 11 patients. We found major differences in the gene-expression levels of androgen, insulin, estrogen, and oxysterol signaling between PCa tissue and cell lines, and between different cell lines. Our systematic characterization gives researchers a solid basis to choose the appropriate PCa cell model for the hormone pathway of interest.

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Sigma-Aldrich
CWR-R1ca Human Prostate Cancer Cell Line, CWR-R1ca Human Prostate Cancer Cell Line is a valuable cell model for castration-recurrent prostate cancer studies.
Sigma-Aldrich
L-(−)-Dithiothreitol, ≥95% (titration)
Sigma-Aldrich
Fetal Bovine Serum, USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Human Prostate Epithelial Cells, Adult, This prostate Cell line provides an ideal serum-free culture model, without retinoic acid, for the study of prostate cells when grown in our recommended medium.