G005745

HPLC Analysis of Mannose and Maltose Sugars on Ascentis^® Express HILIC

application for HPLC

Vlastnosti

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U); mobile phase: [A] water; [B] acetonitrile; (25:75, A:B); flow rate: 1.0 mL/min; column temp.: 35 °C; detector: ELSD, 55 °C, 3.5 bar nitrogen; injection: 10 μL
• suitability: application for HPLC
• application(s): food and beverages

Popis

Analysis Note

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany