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TOYOPEARL® AF-rProtein A HC-650F Bulk Media

phase rProtein A, bottle of 100 mL, 45 μm particle size

Synonyma:

TOYOPEARL® AF-rProtein A HC-650F Bulk Media

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About This Item

UNSPSC Code:
41106500

description

Adsorption capacity (> 80 g/L IgG (static); >70 g/L IgG (dynamic at 5 min. residence time))
Clean in Place (CIP) (0.1 M NaOH)
Phase filter (Protein A)

product line

TOYOPEARL®

form

slurry

packaging

bottle of 100 mL

manufacturer/tradename

Tosoh 23426

parameter

3 bar max. pressure

technique(s)

LPLC: suitable

matrix

hydroxylated methacrylic polymer particle platform

matrix active group

rProtein A phase

particle size

45 μm

pore size

1000 Å

pH

2-13

capacity

>70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time)
 >80 g/L adsorption capacity (IgG)(static)

separation technique

affinity

storage temp.

2-8°C

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General description

TOYOPEARL AF-rProtein A HC-650F is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH.
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.

Specifications

Clean in place with 0.1 M NaOH.

Physical form

72% (v/v) slurry in 20% (v/v) ethanol

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

103.1 °F

flash_point_c

39.5 °C

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Lot/Batch Number

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