Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
53569-U
Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns
L × I.D. 10 cm × 2.1 mm, HPLC Column
Synonyma:
Core-shell (SPP) Fused Core PFP HPLC column
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
1 ea | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 23 400,00 Kč |
O této položce
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
10 cm × 2.1 mm
Particle size:
2.7 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
90 Å pore size
Matrix:
Fused-Core particle platform, superficially porous particle
23 400,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohliNázev produktu
Ascentis® Express F5, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
material
stainless steel column
Quality Segment
agency
suitable for ISO 21675 2019, suitable for USP L43
product line
Ascentis®
feature
endcapped
analyte functional class(es)
antibiotics, explosives, fat soluble vitamins, sweeteners, water soluble vitamins
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range, 600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
10 cm × 2.1 mm
surface area
135 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
2.7 μm
pore size
90 Å pore size
operating pH
2-8
application(s)
PFAS testing
food and beverages
separation technique
reversed phase
General description
The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Application
- Interference of Preservatives on Urinary Iodine Measurement by Sandell-Kolthoff Method.: This research examines the impact of preservatives, including hydrochloric acid, on the accuracy of urinary iodine measurements. The study highlights the need for careful selection of preservatives to avoid analytical interference (Kaya et al., 2022).
- Monitoring the Extraction of Copper from Chicken Dung Leachate Using an Aluminium Electrode as an Indicator.: The study explores the use of hydrochloric acid in the leaching process of copper from chicken dung. It demonstrates the effectiveness of hydrochloric acid in extracting metals from organic waste materials (Kugeria et al., 2019).
- Development of an Analytical Method for Urocanic Acid Isomers in Fish Based on Reactive Extraction Cleanup and Chaotropic Chromatography Techniques.: This paper details a method using hydrochloric acid for the extraction and analysis of urocanic acid isomers in fish. The method enhances the detection and quantification of these compounds in biological samples (Zhong et al., 2018).
- Determination of Single-Ion Activities of H+ and Cl- in Aqueous Hydrochloric Acid Solutions by Use of an Ionic Liquid Salt Bridge.: The research provides a novel approach to determining the activities of hydrogen and chloride ions in hydrochloric acid solutions using an ionic liquid salt bridge, contributing to the understanding of ion behavior in aqueous solutions (Sakaida et al., 2011).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Explore all of our products under Ascentis® Express F5, 2.7 μm HPLC Column
Skladovací třída
11 - Combustible Solids
Co se děje?
WGK 3
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
Vyberte jednu z posledních verzí:
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
-
Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?
1 answer-
Helpful?
-
-
What is the pressure rating for Ascentis® Express F5 HPLC Columns?
1 answer-
Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).
Helpful?
-
-
How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Helpful?
-
-
Can Ascentis® Express F5 HPLC columns be used for LC-MS?
1 answer-
Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis® Express F5 HPLC Columns for high throughput UHPLC and LC-MS are that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
Helpful?
-
-
Are guard columns available for Ascentis® Express F5 2.1 mm ID HPLC Columns?
1 answer-
Yes, we have guard columns available for Ascentis® Express F5 HPLC Column . Product 53500-U is the Ascentis® Express Guard Holder and Product 53594-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 2.1 mm .
Helpful?
-
-
Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?
1 answer-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
Helpful?
-
-
What is the carbon load for Ascentis® Express F5 HPLC Columns?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.
Helpful?
-
-
Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?
1 answer-
Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
Helpful?
-
-
What flow rate should I use with Ascentis® Express F5 HPLC columns?
1 answer-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis® Express F5 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-