ZRB1461

Anti-SLC19A1 Antibody, clone 2E11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyma: Reduced folate transporter;FOLT;Intestinal folate carrier 1;IFC-1;Placental folate transporter;Reduced folate carrier protein;RFC;hRFC;Reduced folate transporter 1;RFT-1;Solute carrier family 19 member 1;hSLC19A1
• UNSPSC Code: 12352203
• NACRES: NA.41

Vlastnosti

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2E11, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 64.87 kDa; observed mol wt ~68 kDa
• purified by: using Protein A
• species reactivity: human, monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001339441
• UniProt accession no.: P41440
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Popis

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2E11 is a Rabbit recombinant monoclonal antibody that specifically detects Reduced folate transporter (SLC19A1). It targets an epitope within 25 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal, cytoplasmic domain of human Reduced folate transporter (SLC19A1).

Application

Quality Control Testing

Evaluated by Western Blotting in Human Placenta tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SLC19A1 in Human Placenta tissue lysate.

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLC19A1 in COS-7 cells.

Affinity Binding Assay: A representative lot of this antibody bound SLC19A1 with a KD of 2.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Reduced folate transporter (UniProt: P41440; also known as FOLT, Intestinal folate carrier 1, IFC-1, Placental folate transporter, Reduced folate carrier protein, RFC, hRFC, Reduced folate transporter 1, RFT-1, Solute carrier family 19 member 1, hSLC19A1) is encoded by the SLC19A1 (also known as FLOT1, RFC1) gene (Gene ID: 6573) in human. SLC19A1 is a multi-pass membrane protein with seven cytoplasmic domains, twelve transmembrane domains, and six extracellular domains. It is highly expressed in the liver and placenta. It serves as a transporter that mediates the import of reduced folate and a subset of immunoreactive cyclic nucleotides. It displays high affinity for N5-methyltetrahydrofolate (Km = 2-7 mM), the predominant circulating form of folate and reduced affinity for folic acid (Km = 150-200 mM). It is also involved in the import of methotrexate. Mechanistically, it acts as an antiporter that exports organic anions to facilitate the uptake of substrates. It operates optimally at pH 7.4, and its activity and folate concentrating ability is reduced with the increase in pH. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Witt, TL., et al. (2004). J. Biol. Chem. 279(45); 46755-46763).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable