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SRP5013

Sigma-Aldrich

CK1 α 1, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonyma:

CK1, CK1A1, CSNK1A1, HLCDGP1, PRO2975

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

102-138 nmol/min·mg

mol wt

~62 kDa

NCBI accession no.

NM_001892

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CSNK1A1(1452)

General description

Casein kinase 1 α1 (CK1α1) is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes. CK1α1 can regulate Smo cell surface accumulation and activity in response to hedgehog. CK1α1 phosphorylates Smo at several sites and phosphorylation-deficient forms of Smo fail to accumulate on the cell surface and are unable to transduce the hedgehog signal. CK1α1 dynamically associates with the CARMA1-BCL10-MALT1 (CBM) complex on T cell receptor engagement to participate in cytokine production and lymphocyte proliferation. CK1α1 can form complex with the E3 ubiquitin-protein ligase MDM2, which then regulates the stability of p53 and E2F-1 transcription factors.

Application

GST tagged active CK1α1 has been used in downstream coprecipitation and mass spectrometry to demonstrate the phosphorylation of the Wnt regulator, Jade-1, by CK1α1.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Osvědčení o analýze (COA)

Lot/Batch Number
Y4158-11
E257-2

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Navštívit knihovnu dokumentů

Oncogenic RAS-induced CK1a drives nuclear FOXO
proteolysis
F. Zhang
Oncogene, 37 (2018)
Fused?Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation
Genes & Development (2007)
Casein Kinase 1 a Phosphorylates the Wnt Regulator Jade-1 and Modulates Its Activity*
Lori Borgal
The Journal of Biological Chemistry (2014)
AIP augments CARMA1-BCL10-MALT1 complex formation to facilitate NF-?B signaling upon T cell activation
Cell communication and signaling : CCS (2014)
Jianhang Jia et al.
Nature, 432(7020), 1045-1050 (2004-12-24)
The Hedgehog (Hh) family of secreted proteins governs cell growth and patterning in animal development. The Hh signal is transduced by the seven-transmembrane protein Smoothened (Smo); however, the mechanism by which Smo is regulated remains largely unknown. Here we show
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