SRP0119

Sirtuin 5 human

recombinant, expressed in E. coli, ≥50% (SDS-PAGE)

• Synonyma: NAD-dependent ADP-ribosyltransferase sirtuin-5, SIR2L5, SIRT5, sir2-like 5
• UNSPSC Code: 12352200
• NACRES: NA.32

Vlastnosti

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥50% (SDS-PAGE)
• form: aqueous solution
• mol wt: 59.8 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_012241
• UniProt accession no.: Q9NXA8
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SIRT5(23408)

Popis

General description

The sirtuin 5 (SIRT5) gene with eight exons is mapped to human chromosome 6p23. The gene exists in two isoforms codings for 310 amino acid and 299 amino acid protein, respectively. Sirtuin 5 is mainly present in heart muscle cells and in lymphoblasts. It is a member of the silent information regulator 2 (Sir2) family of sirtuin histone deacetylases (HDACs). Sirtuin 5 is a mitochondrial protein.
Human Sirtuin 5, GenBank Accession No. NM_012241), full length with N-terminal ST tag, MW = 59.8kDa, expressed in Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Sirtuin 5 (SIRT5) catalyzes the degradation of negatively charged acylated substrates. It also possesses desuccinylase, demalonylase and deglutarylase activities. SIRT5 is a nicotinamide adenine dinucleotide (NAD⁺)-dependent deacetylase and is overexpressed in non-small cell lung cancer (NSCLC) and may have a role in cancer growth. Members of sirtun family play a vital role in epigenetic gene silencing, DNA repair and recombination, cell-cycle, microtubule organization, and in the regulation of aging. SIRT5 has been involved in regulation of the carbamoyl phosphase synthase 1 activity, an essential enzyme of urea cycle, via maintaining lysine glutarylation levels. Sirtuin 5 as a mitochondrial protein plays a major role in controlling ATP production, apoptosis, and cell signaling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable