SAB4503053

Anti-Ubiquitin antibody produced in rabbit

affinity isolated antibody

• Synonyma: RPS27A, UBA52, UBA80, UBCEP1, Ubiquitin
• UNSPSC Code: 12352203
• NACRES: NA.41

Vlastnosti

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 8 kDa
• species reactivity: mouse, rat, human
• concentration: ~1 mg/mL
• technique(s): ELISA: 1:20000; immunohistochemistry: 1:50-1:100
• NCBI accession no.: P62988
• UniProt accession no.: P62987
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... UBA52(7311)

Popis

General description

Anti-Ubiquitin Antibody detects endogenous levels of total Ubiquitin protein.

Ribosomal protein S27a (RPS27A), also known as ubiquitin carboxyl extension protein 1 (UBCEP-1), is a ribosomal protein, which is naturally synthesized as an ubiquitin (Ub) C-terminal extension protein. The protein is encoded by RPS27A gene mapped to human chromosome 2p16. RPS27 is a 9.8kDa protein characterized with one zinc finger domain in its monomeric state.

Immunogen

The antiserum was produced against synthesized peptide derived from human Ubiquitin.

Immunogen Range: 40-89

Application

Anti-Ubiquitin antibody produced in rabbit has been used in following assays:

• Western blot analysis.
• Immunofluorescence-proximity ligation assay (IF-PLA).
• Immunofluorescence.

Biochem/physiol Actions

Ribosomal protein S27a (RPS27a) is involved in ribosome biogenesis and post-translational modifications of proteins. The encoded protein enhances cell proliferation, controls cell cycle progression, and inhibits apoptosis of leukemia cells. Overexpression of RPS27A or ubiquitin results in advanced-phase chronic myeloid leukemia (CML) and acute leukemia (AL). Hence drugs targeting RPS27a combining with tyrosine kinase inhibitor (TKI) might be a potential treatment for TKI resistant CML patients. Elevated expression of ubiquitin carboxyl extension protein 1 (UBCEP-1), which is a component of ubiquitin pathway, promotes prostate carcinoma development, via increasing the degradation of proteins involved in growth inhibition or apoptosis. RPS27a might act as a unique stress sensor in cells, which results in increased p53 activity leading to inhibition of cell cycle progression.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable