SAB4200146

Anti-AMSH-LP / STAMBPL1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody

• Synonyma: Anti-ALMalpha, Anti-AMSH-FP, Anti-AMSH-like protease, Anti-STAM binding protein-like 1, Anti-associated molecule with the SH3 domain of STAM (AMSH) like protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Vlastnosti

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: monkey, canine, human, rat, bovine, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using human HeLa cells; western blot: 2.5-5.0 μg/mL using whole extracts of mouse NIH-3T3 cells; western blot: 5-10 μg/mL using using whole extracts of rat NRK cells
• UniProt accession no.: Q96FJ0
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STAMBPL1(57559) ; mouse ... Stambpl1(76630)

Popis

General description

AMSH-like protein (AMSH-LP), also known as STAM-binding protein-like 1 (STAMBPL1), is a close homolog of AMSH (Associated Molecule with the SH3 domain of STAM). AMSH and AMSH-LP belong to the JAMM (JAB1/MPN/Mov34 metalloenzyme) domain metalloprotease family of Zn²⁺-dependent deubiquitinating enzymes (DUBs). AMSH-LP contains a nuclear localization signal (NLS), an Mpr/Pad1/N-terminal (MPN) domain, and a Jab1/MPN domain metalloenzyme (JAMM) motif.

Application

Anti-AMSH-LP / STAMBPL1 (C-terminal) antibody has been used in

• immunoblotting
• immunofluorescence
• western blotting

Biochem/physiol Actions

AMSH-like protein (AMSH-LP) and Associated Molecule with the SH3 domain of STAM (AMSH) proteins are involved in the deubiquitination of endosomal proteins and specifically cleave K-63-linked (Lys-63) polyubiquitin chains. AMSH-LP, interacts with clathrin heavy chain and this interaction is essential for its endosomal localization. AMSH-LP, interacts with clathrin heavy chain and this interaction is essential for its endosomal localization. It also positively regulatestransforming growth factor beta (TGF-β) signaling through interaction with inhibitory I-SMADs (inhibitory-Mothers against decapentaplegic).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable