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P8169

Phytagel

BioReagent, suitable for plant cell culture, powder

Phytagel™

Synonyma:

Gellan Gum, Agar substitute gelling agent

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Vám/Skladová položkaDostupnostCena
100 g
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1 750,00 Kč
250 g
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5 050,00 Kč
500 g
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8 650,00 Kč
1 kg
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16 400,00 Kč
5 kg
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43 700,00 Kč

O této položce

Číslo CAS:
UNSPSC Code:
10171502
EC Number:
275-117-5
NACRES:
NA.72

1 750,00 Kč


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product line

BioReagent

Quality Segment

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

General description

Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.

Application

Phytagel has been used:
  • in the solidification of culture medium for root assays in Arabidopsis thaliana
  • as a component of Solid Paul′s medium for Agrobacterium culture
  • as a component of root induction medium in Ilex dumosa tissue culture

Biochem/physiol Actions

Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.

Preparation Note

To prevent clumping, add slowly to rapidly stirring medium prior to heating.

Other Notes

An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.

Legal Information

Phytagel is a trademark of Sigma-Aldrich Co. LLC

Porovnat podobné položky

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Zobrazit rozdíly

1 of 1

Tato položka
G1910A9799A4550
Agar High gel strength, suitable for plant cell culture

Sigma-Aldrich

A9799

Agar

Agar Type A, suitable for plant cell culture

Sigma-Aldrich

A4550

Agar

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

form

powder

form

-

form

powder

form

powder

technique(s)

cell culture | plant: suitable

technique(s)

-

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioReagent

product line

-

product line

-

product line

-


Skladovací třída

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  
  1. What is the sulfate content, if any, of Phytagel?

    1 answer
    1. Elemental analysis of this material has not been performed. The sulfate content is not determined.

      Helpful?

  2. Buongiorno. Vorrei sapere se il Phytagel è Gellano ad alto o a basso grado di acilazione

    1 answer
    1. This product is characterized as having a low acyl content.

      Helpful?

  3. What is the molecular weight of one repeating unit?

    1 answer
    1. The molecular weight is not determined. However, the constituent sugars of Phytagel are glucose, glucuronic acid and rhamnose in the molar ratio of 2:1:1.

      Helpful?

  4. What is the gelling strength of Phytagel P8169

    1 answer
    1. The Gel Strength for Phytagel (P8169) is 700 g/cm2 (tested at 0.5%).

      Helpful?

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