P6244
Protein Tyrosine Phosphatase 1B human
recombinant, expressed in E. coli
Synonyma:
PTP-1B
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
Doporučené produkty
recombinant
expressed in E. coli
Quality Level
form
solution
specific activity
≥20 units/mg protein
mol wt
37.4 kDa
concentration
≥0.5 mg/mL
UniProt accession no.
relevant disease(s)
diabetes; obesity; cancer
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTPN1(5770)
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General description
Protein tyrosine phosphatase 1B human (PTP1B) is encoded by the gene PTPN1. In human chromosome, the gene PTPN1 is localized on 20q13.13. The protein has 37 kDa catalytic domain and the C-terminal hydrophobic residues targets the protein to the cytoplasmic side of the endoplasmic reticulum membrane.
Application
Protein Tyrosine Phosphatase 1B (PTP1B) human has been used in treating cell lysate prior to immunoprecipitation and protein pull-down studies. It has also been used in PTP1B activity assay and also for the evaluation of inhibitors like betulinic acid (BA) and sodium orthovanadate (Na3VO4), and 6-chloro-3-formyl-7-methylchromone (CFM) on PTP1B.
Protein tyrosine phosphatase 1B (PTP1B) is a pharmaceutical target used to study the treatment of various diseases such as obesity, cancer and type II diabetes mellitus.
The inhibitory effect of 8-(4-chlorophenylthio)-cAMP and 8-(4-chlorophenylthio)-cGMP on protein tyrosine phosphatase-1B has been assayed.
Biochem/physiol Actions
PTP-1B is a recombinant, non-transmembrane protein expressed in E. coli containing amino acid residues 1-322 of human PTP-1B. It has a molecular mass of 37.4 kDa. It is an abundant intracellular enzyme that is thought to act as a negative regulator of certain signaling pathways. It dephosphorylates tyrosine-phosphorylated proteins and peptides, and is usually localized in the cytostolic domain of the ER.
PTP1B dephosphorylates c-Src in several human breast cancer cell lines has a regulatory role for PTP1B in the control of c-Src kinase activity. PTP1B is regulates the cardiovascular effects of leptin. PTP1B controls the JAK/STAT signaling pathway by dephosphorylation of JAK2.
Polymorphism in the gene PTPN1 is associated with type 2 diabetes and obesity.
Unit Definition
One unit will hydrolyze 1.0 μmole of a phosphopeptide, EGFR fragment 988-998, per minute at pH 7.2 at 30 °C
Physical form
Supplied as a solution in 50 mM HEPES (pH 7.2), 1 mM EDTA, 5 mM DTT, and 0.05% NP-40
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
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Zákazníci si také prohlíželi
Analysis of common PTPN1 gene variants in type 2 diabetes, obesity and associated phenotypes in the French population
Cheyssac C, et al.
BMC Medical Genetics, 7(1), 44-44 (2006)
p66Shc couples mechanical signals to RhoA through FAK-dependent recruitment of p115-RhoGEF and GEF-H1
Wu RF, et al.
Molecular and Cellular Biology, MCB-00194 (2016)
PTP1B: from the sidelines to the front lines!
Tonks NK
Febs Letters, 546(1), 140-148 (2003)
p66Shc couples mechanical signals to RhoA through FAK-dependent recruitment of p115-RhoGEF and GEF-H1
Wu RF, et al.
Molecular and cellular biology, MCB-00194 (2016)
Shaowei Wang et al.
Frontiers in microbiology, 12, 715773-715773 (2021-08-03)
Human PTEN, a dual-phosphatase tumor suppressor, is frequently dysregulated by alternative splicing. Fungi harbor PTEN homologs, but alternative splicing of fungal PTENs has not been reported as far as we know. Here, we described an alternative splicing case in the
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