P1868

Protein Phosphatase 2A₂ from bovine kidney

buffered aqueous glycerol solution

• Synonym(s): PP2A₂
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ~2.0 U/vial
• Biological source: bovine kidney

Properties

• biological source: bovine kidney
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• specific activity: ~2.0 U/vial
• mol wt: dimer 101 kDa (65 kDa and 36 kDa)
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Protein phosphatase 2A (PP2A) is a specific protamine-kinase-inactivating phosphatase, one common physiological form of which is PP2A₂. [1]

Application

Protein phosphatase 2A₂ has been used in a study to investigate two heat-stable protein inhibitors. [2] It has also been used in a study to describe the purification and properties of a protamine kinase from bovine kidney microsomes. [3]

Biochem/physiol Actions

Protein Phosphatase 2A2 from bovine kidney was shown to be a unique inhibitor of protamine kinase while other phosphatases in the same family including PP1, PP2B, PP2C did not show any inhibition. [1]

Physical form

Solution of 1 μg per vial in 50 μl of 50 mM Tris-HCl, pH 7.0, containing 14 mM β-Mercaptoethanol, 1mM benzamidine, 0.1 mM PMSF, 1mM EDTA, and 50% glycerol.

Other Notes

One unit will release 1 nanomole of inorganic phosphate from (32)P-labeled phosphorylase per minute at pH 7.0 at 30 deg C.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable