Přejít k obsahu
Merck
Všechny fotografie(3)

Key Documents

View All Documentation

P0609

Sigma-Aldrich

Pepsin−Agarose from porcine gastric mucosa

lyophilized powder, ≥30 units/mg dry solid

Synonyma:

Pepsin A

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Všechny fotografie(3)

About This Item

Číslo enzymu podle klasifikace EK:
3.4.23.1 (BRENDA, IUBMB)
MDL number:
MFCD00081840
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Porcine gastric mucosa

form

lyophilized powder

specific activity

≥30 units/mg dry solid

mol wt

35 kDa

matrix

4% cross-linked beaded agarose

UniProt accession no.

P00791

storage temp.

−20°C

Gene Information

pig ... LOC396892(396892)

General description

Pepsin is an enzyme found in gastric secretion.

Application

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.
Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.
Used to produce F(ab′)2 fragments of antibodies.1

Biochem/physiol Actions

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

Unit Definition

One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

Quantity

One gram solid swells to 5-10ml packed gel.

Physical form

Lyophilized powder stabilized with lactose

Inhibitor

Č. produktu
Popis
Stanovení ceny

P5318

Pepstatin A, microbial, ≥90% (HPLC)

P7626

Phenylmethanesulfonyl fluoride, ≥98.5% (GC)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Ludmila Tucková et al.
Journal of leukocyte biology, 71(4), 625-631 (2002-04-03)
Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components
M Feldman et al.
Gastroenterology, 110(4), 1043-1052 (1996-04-01)
Recent studies suggesting that gastric secretion does not decrease with aging included few elderly individuals and measured only acid secretion. The aims of this study were to measure gastric acid and pepsin output in 206 health Americans (age range, 18-98
Thomas A Kraus et al.
The Journal of clinical investigation, 115(8), 2234-2243 (2005-07-26)
To explore the requirement for M cells and the Peyer's patch (PP) in induction of oral tolerance and address the potential in vivo role of intestinal epithelial cells as nonprofessional APCs, we have attempted to induce tolerance in mice with
Widya A Wahab et al.
International journal of experimental pathology, 97(4), 303-309 (2016-09-24)
Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin
K L Bøgh et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 39(10), 1611-1621 (2009-08-20)
Food allergies are a public health issue of growing concern, with peanuts in particular being associated with severe reactions. The peanut allergen, Ara h 1, belongs to the cupin plant food allergen family, which, unlike other structural families, appears to
Zobrazit všechny související dokumenty

Sortimentní položky

Antibody Fragmentation with Pepsin Digestion

Antibody fragmentation with our pepsin digestion protocol for IgG antibody fragmentation and preparation of F(ab’).

Protokoly

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.