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MAK088

Sigma-Aldrich

DPP4 Activity Assay Kit

sufficient for 100 fluorometric tests

Synonyma:

CD26 Activity Assay Kit, Deaminase Complexing Protein 2 Activity Assay Kit, Dipeptidyl Peptidase-4 Activity Assay Kit

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

storage temp.

−20°C

Gene Information

human ... DPP4(1803)
mouse ... DPP4(13482)
rat ... DPP4(25253)

General description

Dipeptidyl peptidase-4 (DPP4), also known as CD26 and adenosine deaminase complexing protein 2, is a serine exopeptidase that cleaves X-Proline and X-Alanine residues from the N-termini of polypeptides. DPP4 is a transmembrane glycoprotein whose activity regulates the bioactivity of multiple peptides such as growth factors, chemokines, and neuropeptides. DPP4 plays a major role in glucose metabolism via the regulation of glucagon-like peptide-1 and inhibitors of DPP4 are commonly used for the treatment of type 2 diabetes. DPP4 also plays an important role in immune regulation and may play a role in tumor suppression. Deletion of the DPP4 gene affects carbohydrate metabolism which in turn leads to mental retardation and hypotonia. Abnormal expression of the protein is observed during liver regeneration, cirrhosis and liver tumorigenesis.

Application

DPP4 Activity Assay Kit has been used to determine the activity of dipeptidyl peptidase-4 (DPP4) in homogenates.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of dipeptidyl peptidase-4 (DPP4) in a variety of samples including tissue, cells and serum

Principle

The DPP4 Activity Assay kit provides a simple and direct procedure for measuring DPP4 activity in a variety of samples. In this assay, DPP4 cleaves a non-fluorescent substrate, H-Gly-Pro-AMC, to release a fluorescent product, 7-Amino-4-Methyl Coumarin (AMC) (λex = 360/λem = 460 nm). One unit of DPP4 is the amount of enzyme that will hydrolyze the DPP4 substrate to yield 1.0 μmole of AMC per minute at 37 °C.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Osvědčení o analýze (COA)

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Eri Watanabe et al.
Angiogenesis, 23(4), 667-684 (2020-07-24)
Platelet-derived growth factor-B (PDGF-B) is a main factor to promote adipose tissue angiogenesis, which is responsible for the tissue expansion in obesity. In this process, PDGF-B induces the dissociation of pericytes from blood vessels; however, its regulatory mechanism remains unclear.
Molecular analyses of human and rat dipeptidyl peptidase IV.
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Cellular peptidases in immune functions and diseases, 161-169 (1997)
Active phytochemicals of Pueraria tuberosa for DPP-IV inhibition: in silico and experimental approach.
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Journal of diabetes and metabolic disorders, 16(1), 46-46 (2017)
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Frontiers in pharmacology, 12, 779652-779652 (2021-12-25)
Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease and has become a serious public health problem worldwide. Dipeptidyl peptidase-4 (DPP4) inhibitors, an emerging drug for the treatment of diabetes, have been found to have renoprotective effects
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Srivastava S, et al.
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