M9763

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate

fluorogenic, ≥98% (TLC), powder

• Synonyma: 4-Methylumbelliferyl-N,N′-diacetyl-β-D-chitobioside hydrate
• Empirický vzorec (Hillův zápis): C₂₆H₃₄N₂O₁₃ · xH₂O
• Číslo CAS: 53643-12-2
• Molekulová hmotnost: 582.55 (anhydrous basis)
• MDL number: MFCD00082499
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897364
• NACRES: NA.28

Vlastnosti

• product name: 4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate, ≥98% (TLC)
• Quality Level: 200
• assay: ≥98% (TLC)
• form: powder
• solubility: DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow
• fluorescence: λ_ex 330 nm; λ_em 375 nm in methanol
• storage temp.: −20°C
• SMILES string: CC(=O)NC1C(O)C(O)C(CO)OC1OC2C(O)C(NC(C)=O)C(OC2CO)Oc3ccc4C(C)=CC(=O)Oc4c3
• InChI: 1S/C26H34N2O13/c1-10-6-18(33)38-15-7-13(4-5-14(10)15)37-25-20(28-12(3)32)23(36)24(17(9-30)40-25)41-26-19(27-11(2)31)22(35)21(34)16(8-29)39-26/h4-7,16-17,19-26,29-30,34-36H,8-9H2,1-3H3,(H,27,31)(H,28,32)
• InChI key: UPSFMJHZUCSEHU-UHFFFAOYSA-N

Popis

Application

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate has been used as a fluorogenic substrate:

• in chitinase enzyme assay of bronchoalveolar lavage samples
• in acidic mammalian chitinase activity assay of human gastric juice
• in chitinase assay in crystalline style extracts of Greenshell Mussels and fungus Fusarium oxysporum.

Biochem/physiol Actions

4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate (MUF-diNAG) is an analog of chitin. It is a fluorogenic substrate for chitin depolymerase and its breakdown results in methylumbelliferone, a fluorescent product.

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)