Přejít k obsahu
Merck
Všechny fotografie(1)

Key Documents

View All Documentation

L9379

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder

Synonyma:

PWM, Pokeweed mitogen

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Všechny fotografie(1)

About This Item

MDL number:
MFCD00161973
UNSPSC Code:
12352202
NACRES:
NA.32

sterility

aseptically filled

Quality Level

200

form

lyophilized powder

potency

≤0.3 μg/mL mitogenic activity

storage temp.

2-8°C

Hledáte podobné produkty? Navštivte Průvodce porovnáváním produktů

General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Lectin from Phytolacca americana (pokeweed) has been used:
  • as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation
  • as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes
  • as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures

Biochem/physiol Actions

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Physical form

Partially purified TCA precipitate.
Essentially salt-free.

Analysis Note

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism
Demyda P, et al.
Theriogenology, 81(8), 1116-1122 (2014)
Assessment of cellular immune responses of healthy and diseased Tasmanian devils (Sarcophilus harrisii)
Kreiss A, et al.
Developmental and Comparative Immunology, 32(5), 544-553 (2008)
B Hejdeman et al.
HIV medicine, 4(2), 101-110 (2003-04-19)
To study the impact of effective highly active antiretroviral therapy (HAART) on the preservation of long-term CD4 memory cells induced by vaccines in HIV-1-infected patients. Thirty HIV-1-positive patients on HAART with undetectable viral load were randomized into three groups: 10
Nishaki Mehta et al.
Vaccine, 28(21), 3672-3678 (2010-04-02)
Hepatitis B-specific memory B cell (HSMBC) frequencies were measured following hepatitis B vaccination in 15 HIV uninfected and 12 HIV infected adolescents. HSMBC were detected at significantly lower frequencies in HIV infected than in HIV uninfected individuals. The detection of
The immune response of the Tasmanian devil (Sarcophilus harrisii) and devil facial tumour disease
Woods GM, et al.
EcoHealth, 4(3), 338-345 (2007)
Zobrazit všechny související dokumenty

Sortimentní položky

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.