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I3146

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonyma:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

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Vám/Skladová položkaDostupnostCena
5 mL
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2 690,00 Kč

O této položce

NACRES:
NA.75
UNSPSC Code:
12352205

2 690,00 Kč


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sterility

sterile-filtered

Quality Segment

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells[1]
  • rat fetal testis explants[2]
  • human dental pulp stem cells (DPSC)[3]

Biochem/physiol Actions

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.[4]
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.[5]

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Other Notes

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

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Tato položka
I2521I2771I0516
form

liquid

form

liquid

form

liquid

form

solution

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

500

Quality Level

300

Quality Level

400


Skladovací třída

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Lot/Batch Number

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Questions

1–5 of 5 Questions  
  1. 培地で希釈した1×Solutionは冷蔵でどのくらい保管できますか?

    1 answer
    1. The 100x stock solution of this product has a 2-year shelf life when stored at 2-8°C. However, stability of the solution after dilution or after opening has not been tested.

      Helpful?

  2. Can this ITS be diluted in DMEM medium containing phenol red

    1 answer
    1. This product is compatible with DMEM containing phenol red.

      Helpful?

  3. What should you use to dilute this to a 1X solution?

    1 answer
    1. This product can be dissolved in a given target cell culture medium, e.g., non-complex media (MEM, RPMI-1640), to a 1X solution.

      Helpful?

  4. What are the product numbers for the stand alone components in I3146?

    1 answer
    1. The product numbers for the stand-alone components in I3146 are as follows:
      Insulin – I2643 or I3536 (sterile)
      Apo-Transferrin – T2252
      Sodium Selenite – S5261
      EBBS – E3024

      Helpful?

  5. The ITS product #I3146 has been on back order for a while. Is there an alternative with similar components?

    1 answer
    1. Based on the Media Supplement Formulation, the closest alternative to product No. I3146 is the I2521 ITS+1 Liquid Media Supplement (100×), which also contains BSA and Linoleic acid.

      Helpful?

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