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Key Documents

GE28-9852-54

rProtein A GraviTrap™

Cytiva 28-9852-54, pack of 10 × 1 mL

Synonyma:

GraviTrap

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

packaging

pack of 10 × 1 mL

manufacturer/tradename

Cytiva 28-9852-54

storage condition

(20% Ethanol)

bed volume

1 mL

capacity

50 mg binding capacity (human IgG/column)(Protein binding capacity is protein-to-protein dependent.)

storage temp.

2-8°C

General description

rProtein A GraviTrap™ includes ten ready-to-use columns prepacked with 1 mL of rProtein A Sepharose™ Fast Flow.

Application

rProtein A GraviTrap™ are prepacked gravity-fLow columns designed for fast and efficient manual purification of monoclonal and polyclonal antibodies, antibody fragments from cell culture supernatant, and biological fluids. The antibodies are simply captured with high specificity on Protein A ligands in gravity-fLow columns. You do not need any other instrument with this protocol because the entire process relies on the flow of gravity. The yield varies from 20 to 50 mg of purified antibodies depending on the ligand you use. The columns are reusable up to five times.

Features and Benefits

  • Prepacked gravity-fLow columns provide convenience, save time, and increase the reliability of your results.
  • Purification of antibodies in 30 min allows you to quickly obtain and store your target Protein in order to avoid the inherent dangers of Protein instability and degradation associated with sLow and long purification runs.
  • High purity and recovery with purity > 95% in one step and recovery typically 70% to 80%.
  • High binding capacity such as rProtein A GraviTrap™ ~ 50 mg human IgG/column

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

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Performing a Purification of IgG Antibodies with Protein G GraviTrap™

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