GE28-9782-45
HiScreen™, DEAE Fast Flow
Cytiva 28-9782-45
About This Item
Doporučené produkty
ligand
diethylaminoethyl
description
Ion Exchanger Type (value)
form
prepacked column
crosslinking
cross-linked
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9782-45
storage condition
(20% Ethanol)
parameter
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
technique(s)
liquid chromatography (LC): suitable
bed size
7.7 mm × 100 mm
bed volume
4.7 mL
column I.D.
7.7 mm
matrix
6% cross-linked agarose
matrix active group
N+(C2H5)2H functional group
particle size
45-165 μm
average diameter
90 μm
cleaning
2-14
working range
2-12
capacity
binding capacity(~ 110 mg HAS/mL resin)
suitability
suitable for bioprocess medium
separation technique
anion exchange
General description
Application
If necessary, two columns can easily be connected in series to give a bed height of 20 cm.
Media prepacked in HiScreen™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.
Features and Benefits
- DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
- Prepacked, ready-to-use columns for convenient process development optimization.
- 10 cm bed height of HiScreen™ columns is designed to allow method optimization and parameter screening.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
Storage and Stability
Analysis Note
Legal Information
related product
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Sortimentní položky
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protokoly
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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