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G8541

Sigma-Aldrich

Ala-Gln

200 mM, solution, sterile-filtered, Biotechnology Performance Certified

Synonyma:

Alanyl-glutamine, Glutamine-S

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

Empirický vzorec (Hillův zápis):
C8H15N3O4
Číslo CAS:
39537-23-0
Molekulová hmotnost:
217.22
MDL number:
MFCD00133046
UNSPSC Code:
12352209
PubChem Substance ID:
24895343
NACRES:
NA.75

grade

Biotechnology Performance Certified

sterility

sterile-filtered

form

solution

concentration

200 mM

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

NC(CC[C@H](NC([C@@H](N)C)=O)C(O)=O)=O

InChI

1S/C8H15N3O4/c1-4(9)7(13)11-5(8(14)15)2-3-6(10)12/h4-5H,2-3,9H2,1H3,(H2,10,12)(H,11,13)(H,14,15)/t4-,5-/m0/s1

InChI key

HJCMDXDYPOUFDY-WHFBIAKZSA-N

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General description

Ala-Gln supplement provides a stable form of L-glutamine while in solution.

Application

Ala-Gln has been used as a supplement in Dulbecco′s Modified Eagle Medium (DMEM) media-rich in glucose and pyruvate to culture luciferase-expressing MDA-MB-231-luc tumor cells to measure the bioluminescence signals of cells leaking during intra-bone marrow transplantation (IBMT) from dissected long bones. It has also been used as a DMEM - high glucose media to culture human cell lines.

Other Notes

Contains the dipeptide L-Alanyl-L-glutamine. 200 mM supplied in 0.85% NaCl.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Dieter Fink et al.
PloS one, 14(11), e0224576-e0224576 (2019-11-08)
Intra-bone marrow transplantation (IBMT) has been adapted for mouse models to improve the seeding efficiency of transplanted hematopoietic stem and progenitor cells. Commonly used injection volumes for IBMT into the tibia differ between 10 and 40 μL even though considerable
Daren K Heyland et al.
JPEN. Journal of parenteral and enteral nutrition, 39(4), 401-409 (2014-05-08)
The recent large randomized controlled trial of glutamine and antioxidant supplementation suggested that high-dose glutamine is associated with increased mortality in critically ill patients with multiorgan failure. The objectives of the present analyses were to reevaluate the effect of supplementation
Marek Bartosovic et al.
Nature biotechnology, 39(7), 825-835 (2021-04-14)
In contrast to single-cell approaches for measuring gene expression and DNA accessibility, single-cell methods for analyzing histone modifications are limited by low sensitivity and throughput. Here, we combine the CUT&Tag technology, developed to measure bulk histone modifications, with droplet-based single-cell
Spyridon T Pachis et al.
Cell reports, 26(8), 2101-2112 (2019-02-21)
Faithful chromosome segregation relies on the ability of the spindle assembly checkpoint (SAC) to delay anaphase onset until chromosomes are attached to the mitotic spindle via their kinetochores. MPS1 kinase is recruited to kinetochores to initiate SAC signaling and is
S Taavitsainen et al.
Nature communications, 12(1), 5307-5307 (2021-09-08)
Prostate cancer is heterogeneous and patients would benefit from methods that stratify those who are likely to respond to systemic therapy. Here, we employ single-cell assays for transposase-accessible chromatin (ATAC) and RNA sequencing in models of early treatment response and
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