G1512
β-Glucuronidase from Helix pomatia
Type H-5, lyophilized powder, ≥400,000 units/g solid
Synonyma:
β-D-Glucuronide glucuronosohydrolase
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
100000 units | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 930,00 Kč |
500000 units | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 10 100,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
≥400,000 units/g solid
2 930,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
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Type H-5
Quality Segment
form
lyophilized powder
specific activity
≥400,000 units/g solid
secondary activity
≤40,000 units/g solid sulfatase
storage temp.
−20°C
General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application
β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.[1]
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
β-Glucuronidase from Helix pomatia has been used:
- in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
- in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
- in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)
Biochem/physiol Actions
β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Other Notes
A further purification of G0876 by gel permeation chromatography.
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
1 of 1
Tato položka | |||
|---|---|---|---|
| specific activity ≥400,000 units/g solid | specific activity ≥90,000 units/mL | specific activity ≥100,000 units/mL | specific activity ≥60,000 units/mL |
| form lyophilized powder | form aqueous solution | form aqueous solution | form aqueous solution |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 300 |
| type Type H-5 | type Type HP-2S | type Type HP-2 | type Type H-3AF |
| secondary activity ≤40,000 units/g solid sulfatase | secondary activity ≤7,500 units/mL sulfatase | secondary activity ≤7,500 units/mL sulfatase | secondary activity - |
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signalword
Danger
Skladovací třída
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
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