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G7770

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type HP-2S, aqueous solution, ≥90,000 units/mL

Synonyma:

β-D-Glucuronide glucuronosohydrolase

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About This Item

Číslo CAS:
9001-45-0
Číslo enzymu podle klasifikace EK:
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

sterility

sterile-filtered

Quality Level

200

type

Type HP-2S

form

aqueous solution

specific activity

≥90,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

storage temp.

2-8°C

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General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

β-Glucuronidase from Helix pomatia has been used:
  • in the treatment of heparinized plasma sample for the hydrolysis of glucuronic acid-conjugated metabolites
  • in the pre-treatment of plasma sample for the determination of 1′-hydroxymidazolam (1′-OHMDZ)
  • for the treatment of bile samples from rainbow trouts for the hydrolysis of glucuronate and sulfate metabolites
  • in treating the cells from the sporulation media for the complete digestion of the cell walls of asci

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin. It is used as a reporter gene in GUS assays to monitor gene expression.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

substrate

Č. produktu
Popis
Stanovení ceny

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

Osvědčení o analýze (COA)

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Sortimentní položky

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protokoly

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

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