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F2012

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonyma:

Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL number:
MFCD00162642
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:64 (minimum)

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
The product binds all mouse Igs.

Application

Anti-Mouse IgG (whole molecule)−FITC antibody has been used:
  • in immunofluorescence
  • in immunohistochemistry
  • in immunofluorescence staining
  • in immunofluorescence microscopy
  • in confocal imaging

Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat was used for FACS analysis of DF3 antigen on breast cancer cell lines.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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The
The Laboratory Rat (2005)
L Chen et al.
The Journal of clinical investigation, 96(6), 2775-2782 (1995-12-01)
The high molecular weight mucin-like glycoprotein, DF3 (MUC1), is overexpressed in the majority of human breast cancers. Here we demonstrate that replication defective recombinant adenoviral vectors, containing the DF3 promoter (bp -725 to +31), can be used to express beta-galactosidase
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Baik K Y, et al.
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Journal of virology, 414(2-3), JVI-03130 (2013)
Stimulation of intracellular sphingosine-1-phosphate production by G-protein-coupled sphingosine-1-phosphate receptors
zu H D M, et al.
European Journal of Pharmacology, 414(2-3), 145-154 (2001)
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