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DUO92104

Sigma-Aldrich

Duolink® In Situ Orange Starter Kit Mouse/Goat

Synonyma:

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

product line

Duolink®

Quality Level

100

technique(s)

proximity ligation assay: suitable

fluorescence

λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)

suitability

suitable for fluorescence

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

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Bezpečností list
  • DUO92006Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)Bezpečností list
  • DUO92001Duolink® In Situ PLA® Probe Anti-Mouse PLUSBezpečností list
  • DUO92007Duolink® In Situ Detection Reagents OrangeBezpečností list
  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceBezpečností list
  • DUO82040Duolink® In Situ Mounting Medium with DAPIBezpečností list

signalword

Danger

Hazard Classifications

Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

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Lot/Batch Number

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Protokoly

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This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

Související obsah

Duolink® PLA Applications

Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

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