D5905

3,3′-Diaminobenzidine tetrahydrochloride

peroxidase substrate, chromogenic, tablet

• Synonyma: 3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB
• Lineární vzorec: (NH₂)₂C₆H₃C₆H₃(NH₂)₂ · 4HCl
• Molekulová hmotnost: 360.11
• NACRES: NA.83
• PubChem Substance ID: 24893981
• UNSPSC Code: 12352204
• EC Number: 231-018-9
• MDL number: MFCD00012969
• Beilstein/REAXYS Number: 1212988

Vlastnosti

• Název produktu: 3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• solubility: water: 1 tablet/15 mL (may contain small insoluble particles)
• storage temp.: −20°C
• SMILES string: Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
• InChI: 1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
• InChI key: KJDSORYAHBAGPP-UHFFFAOYSA-N

Popis

Application

Reagent for spectrophotometric determination of selenium.

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.

Biochem/physiol Actions

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Preparation Note

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Other Notes

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Bezpečnostní informace

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302,H319,H341,H350
• pcodes: P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
• Skladovací třída: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• ppe: Eyeshields, Gloves, type N95 (US)