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Key Documents

D0564

Sigma-Aldrich

Diphtheria Toxin

from Corynebacterium diphtheriae, ≥98% (SDS-PAGE), lyophilized powder, protein synthesis inhibitor

Synonyma:

DTx

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

MDL number:
MFCD00163490
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Diphtheria Toxin from Corynebacterium diphtheriae, lyophilized powder, Product is in unnicked form

Quality Level

200

form

lyophilized powder

storage temp.

2-8°C

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General description

Diphtheria toxin may be used as a toxin element in the construction of immunotoxin for cell-specific cytotoxicity. The translocation domain of bacterial toxins with a natural endosome escape mechanism has been used in the development of efficient nonviral vectors for applications in gene therapy.

Application

Diphtheria Toxin from Corynebacterium diphtheriae has been used:


  • for microglia depletion to study post-traumatic stress disorder in mice
  • intraperitoneally injected in transgenic mice to select the hybrids after fusion of transplanted hematopoietic stem and progenitor cells (HSPCs) during ablation
  • to study its effect on eosinophil lineage-committed progenitors in an eosinophil-deficient strain of mice (iPHIL)

Biochem/physiol Actions

Diphtheria toxin is a bacterial toxin produced from Corynebacterium diphtheriae. It contains three domains that have intracellular actions. The domains are involved in the intoxication of the cell, cell-surface binding and internalization into endosomes, translocating into the cytosol across the endosome membrane and inhibiting cellular protein synthesis. Diphtheria toxin is useful in constructing biotechnological tools and therapeutics. It inhibits protein synthesis by catalyzing adenosine diphosphate (ADP)-ribosylation of the target protein in eukaryotes.

Physical form

Lyophilized powder containing Tris and EDTA.

Reconstitution

Each vial, when reconstituted to 0.5 mL with sterile distilled water, contains ~1 mg of diphtheria toxin in 0.01 M Tris and 0.001 M Na2EDTA, pH 7.5.

Pictograms

Skull and crossbones
GHS06

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 1 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Diphtheria toxin
The Comprehensive Sourcebook of Bacterial Protein Toxins (2015)
Biology and molecular epidemiology of diphtheria toxin and the tox gene
The Journal of Infectious Diseases, 181 (2000)
S M Yellon et al.
Biology of reproduction, 100(5), 1386-1394 (2019-01-11)
To test the hypothesis that macrophages are essential for remodeling the cervix in preparation for birth, pregnant homozygous CD11b-dtr mice were injected with diphtheria toxin (DT) on days 14 and 16 postbreeding. On day 15 postbreeding, macrophages (F4/80+) were depleted
Vicky A Tobin et al.
Nature, 464(7287), 413-417 (2010-02-26)
Many peptides, when released as chemical messengers within the brain, have powerful influences on complex behaviours. Most strikingly, vasopressin and oxytocin, once thought of as circulating hormones whose actions were confined to peripheral organs, are now known to be released
Benjamin J Vakoc et al.
Nature medicine, 15(10), 1219-1223 (2009-09-15)
Intravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability
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