COR-L 23/CPR

96042336, human lung, Not specified

• UNSPSC Code: 41106514

Vlastnosti

• Název produktu: COR-L 23/CPR, 96042336
• biological source: human lung
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Human Caucasian lung, large cell carcinoma

Cell Line Description

The cell line COR-L23/CPR is a drug-resistant variant of COR-L23 (Sigma Catalogue number. 92031919). The line was developed by growing the parent line in increasing concentrations of cisplatin. Cisplatin accumulation is reduced in COR-L23/CPR and cross resistance to melphalan and other platinum compounds has been found. Cells tend to grow in clumps.

Application

Analysis of acquired drug resistance, study of large cell carcinoma of the lung

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,11
D16S539: 11,13
D5S818: 10,12
D7S820: 9
THO1: 9
TPOX: 8
vWA: 19

Culture Medium

RPMI 1640 + 2mM Glutamine + 0.05μg / ml Cisplatin + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5 x 10,000 cells / cm² using 0.25 % trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage.

Other Notes

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