DSL6A/C1

94022431, rat pancreas, Polygonal

• UNSPSC Code: 41106514

Vlastnosti

• product name: DSL6A/C1, 94022431
• biological source: rat pancreas
• growth mode: Adherent
• karyotype: Not specified
• morphology: Polygonal
• products: Expresses CFTR protein
• receptors: Lacks CCK-A receptors
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Rat pancreatic carcinoma

Cell Line Description

DSL6A/C1 were derived from a transplantable acinar cell carcinoma (DSL-6) that had been established from a primary carcinoma of the pancreas in an azaserine treated Lewis rat. The expression of the ductal cell marker OC.2 was shown with a sub-population expressing ductal marker OV-6, CK-7 and CK-19. DSL6A/C1 cells are reported to be tumorigenic in Lewis rats and produce solid tumours composed of duct like structures surrounded by dense fibrous tissue. The cell line lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and re-grafting.

Application

Study of pancreatic carcinomas, differentiation of acinar/ductal cells.

Culture Medium

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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