HTC

93120108, rat liver, Epithelial

• UNSPSC Code: 41106514

Vlastnosti

• product name: HTC, 93120108
• biological source: rat liver
• growth mode: Adherent
• karyotype: 2n = 66, hypertriploid
• morphology: Epithelial
• products: Not specified
• receptors: Dexamethasone phosphate; hydrocortisone
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Rat hepatoma

Cell Line Description

Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N′-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.

Application

Tumourigenicity and enzymatic studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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