LS180

87021202, human colon, Epithelial-like

• UNSPSC Code: 41106514

Vlastnosti

• product name: LS180, 87021202
• biological source: human colon
• growth mode: Adherent
• karyotype: Modal no. 45 (2n=46)
• morphology: Epithelial-like
• products: Carcinoembryonic antigen (CEA), Mucin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

Application

Tumourigenicity studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

Other Notes

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