HT115

85061104, human colon, Epithelial

• Synonyma: HT-115 Cells
• UNSPSC Code: 41106514

Vlastnosti

• product name: HT115, 85061104
• biological source: human colon
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Human colon carcinoma

Cell Line Description

Cells possess Ca antigen as defined by the Ca1 antibody. The Ca antigen has been shown to be absent in non-malignant human cell strains and is considered to be a marker linked with malignancy. Island cell growth will degenerate if left more than 9 days between passages.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 7,12
D13S317: 13
D16S539: 10
D5S818: 12
D7S820: 11,12
THO1: 6,9
TPOX: 8
vWA: 18

Culture Medium

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells grow relatively slowly, in islands.

Other Notes

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