MM537

12070509, human skin, Epithelial

• Synonyma: MM 537
• UNSPSC Code: 41106514

Vlastnosti

• product name: MM537, 12070509
• biological source: human skin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• receptors: Not specified
• relevant disease(s): metastasis

Popis

Cell Line Origin

Human melanoma; from metastatic site - lymph node

Cell Line Description

mM537 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1356.

DNA Profile

Not specified

Culture Medium

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.

Subculture Routine

Optimal split ratio 1:4 (minimum seeding density 2.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 1-2 min. MM537 required growth medium to be changed 3 times each week. The cells are sensitive to trypsin so exposure time to trypsin should be kept to a minimum. The cells take 2-3 days to recover from trypsinisation.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.