SPC212

11120717, human lung, Epitheloid/mesenchymal

• Synonyma: SPC 212, SPC-212
• UNSPSC Code: 41106514

Vlastnosti

• product name: SPC212, 11120717
• biological source: human lung
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epitheloid/mesenchymal
• receptors: Mesothelial-membrane antigen ME1
• relevant disease(s): cancer

Popis

Cell Line Origin

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Cell Line Description

SPC212 was derived from a biphasic tumour of the pleural cavity of a 47-year old female patient, prior to treatment, with a known history of exposure to asbestos.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 11,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16

Culture Medium

DMEM:F12 (1:1) (D8062) + L-Glutamine (G7513) + 15% FBS / FCS (F2442).

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO₂; 37 °C. Suggested seeding density 1-4 x 10,000 cells/cm².

Other Notes

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