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Key Documents

C4562

Sigma-Aldrich

Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse

clone hHCD, ascites fluid

Synonyma:

Anti-CDM, Anti-H-CAD, Anti-HCAD, Anti-L-CAD, Anti-LCAD, Anti-NAG22, Anti-h-CD

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

MDL number:
MFCD00164554
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

hHCD, monoclonal

contains

15 mM sodium azide

species reactivity

sheep, rabbit, human, pig, bovine, mouse

technique(s)

immunohistochemistry: 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
immunoprecipitation (IP): suitable
western blot: 1:4,000 using human uterus extract

isotype

IgG1

UniProt accession no.

Q05682

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CALD1(800)
mouse ... Cald1(109624)

Specificity

The antibody (also cited as h-CD) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-caldesmon). It does not cross-react with skeletal or cardiac muscle or with the 70 kDa non-muscle caldesmon. In immunohistochemical staining, the antibody exhibits smooth muscle specificity. It stains vascular and visceral smooth muscle cells but not epithelial, endothelial or connective tissue fibroblast cells. In normal and malignant breast tissue, the myoepithelial component of galactophorous sinuses (but not in ducts of lobules) is stained; however, the antibody may be non-reactive by immunocytochemical methods with cultured smooth muscle cells. This probably reflects the down regulation of caldesmon commonly observed in tissue culture.

Immunogen

human uterus smooth muscle extract.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Caldesmon (Smooth) antibody produced in mouse is suitable for:
  • immunohistochemistry at a dilution of 1:500 using methacarn-fixed, paraffin-embedded sections of human or animal tissue
  • immunoprecipitation
  • western blot at a dilution of 1:4,000 using human uterus extract

Biochem/physiol Actions

Monoclonal Anti-Caldesmon (Smooth) (mouse IgG1 isotype) reacts in immunoblotting assays with caldesmon polypeptide of 150 kDa (h-Caldesmon).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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