Aqueous solutions of ATP are stable for months when frozen in solution at -15 °C and for approximately one week at 0 °C. As with most products, we guarantee the quality of this material for 1 year when stored unopened at the recommended storage temperature (-20°C in this case).
A6559
Adenosine 5′-triphosphate disodium salt solution
≥95% (HPLC), liquid
Synonyma:
ATP
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
25 μmol | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 900,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
41106305
eCl@ss:
32160414
PubChem Substance ID:
NACRES:
NA.52
MDL number:
Assay:
≥95%
Biological source:
yeast
Form:
liquid
Storage temp.:
−20°C
2 900,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Dovolte nám, abychom vám pomohliNázev produktu
Adenosine 5′-triphosphate disodium salt solution, Crystalline ATP, HPLC purified, aqueous solution for RNA transcription
biological source
yeast
Quality Segment
assay
≥95%
form
liquid
concentration
100 mM in H2O (adjusted to pH 7 with Trizma®
base)
foreign activity
DNase, RNase, none detected
shipped in
dry ice
storage temp.
−20°C
SMILES string
[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
InChI
1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1
InChI key
TTWYZDPBDWHJOR-IDIVVRGQSA-L
General description
Adenosine 5′-triphosphate disodium salt solution is suitable for use in molecular biology applications such as kinase reactions or DNA-dependent RNA polymerase transcription.
Application
Adenosine 5′-triphosphate disodium salt solution has been used:
- as a standard to evaluate the extracellular ATP release from marginal cells of stria vascular[1]
- as a component of the internal pipette solution to establish a recording electrode to study neurite stretching[2]
- in in vitro VPS34 (catalytic subunit of the phosphatidylinositol 3-kinase (PtdIns3K) complex) activity assay
- as a component of lysis and resealing buffers for ATP studies[3]
- in spectrophotometric measurement of H+ transport in fluorescein isothiocyanate (FITC)-dextran loaded A549 (adenocarcinomic human alveolar basal epithelial cells) and HeLa cells[4]
- to induce pyroptosis in lipopolysaccharide treated fibroblast-like synoviocytes (FLSs)[5]
Biochem/physiol Actions
Adenosine 5′-triphosphate (ATP) is an important energy currency in all living organisms. It possesses high phosphate transfer potential. ATP is involved in several biological processes such as membrane transport, muscle contraction and synthesis, and degradation of biological molecules. It plays a role as an intracellular and extracellular signaling molecule in certain cellular processes such as cell motility, organ development, neurotransmission, and insulin secretion.[6]
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tato položka | |||
|---|---|---|---|
| biological source yeast | biological source - | biological source - | biological source synthetic (organic) |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| assay ≥95% | assay ≥99% | assay ≥99% | assay ≥99% |
| concentration 100 mM in H2O (adjusted to pH 7 with Trizma® | concentration 100 mM | concentration 10 mM | concentration 100 mM in H2O |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Skladovací třída
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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How long can aqueous ATP solutions like Product No. A6559 be stored?
1 answer-
Helpful?
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Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used for in vitro transcription?
1 answer-
The purity of this product should be sufficient for use in T7 RNA polymerase-catalyzed in vitro transcription assay, but we do not test the product for use in that application in our quality control process.
Helpful?
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What is meant by 25UMO in the size description of Product A6559, Adenosine 5´-triphosphate disodium salt solution? What is the product dissolved in?
1 answer-
25UMO means 25 micromoles. The 25 UMO size is equivalent to 250 μl of a 100 mM solution. This product is a 100 mM solution in water; the solution pH has been adjusted with Trizma base but contains no additional buffer or salts.
Helpful?
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Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used in molecular biology applications?
1 answer-
This is a Molecular Biology grade ATP. It is certified free of DNase and RNase. This product is recommended for any typical molecular biology application.
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What is the binding affinity of ATP (Product No. A6559) for Mg++ and other metal ions?
1 answer-
ATP has intrinsic metal binding affinity. The binding constant for various metals are (given as per mole): Mg++, 9554; Na+, 13; Ca++, 3722; K+, 8; Sr++, 1381; Li+, 25. The ability of ATP to chelate magnesium results in effective Mg++ ion concentrations lower than the total Mg ion concentration. For example, in a reaction with 1.5mM Mg++ and 0.2 mM ATP, the effective Mg ion concentration is ~1.3mM.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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Can this preparation of Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used as an ATP standard?
1 answer-
This preparation of ATP is not recommended for use as an ATP standard. The recommended ATP products for use as a standard in bioluminescence experiments is Product Code FL-AAS (preweighed vials of 1 mg each = 2 micromole).
Helpful?
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What is the millimolar extinction coefficient for ATP, Product No. A6559?
1 answer-
The millimolar extinction coefficient (EmM) is 15.4, measured at 259 nm for a sample in 100 mM phosphate buffer, pH 7.0.
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