94149
Atto 647N-Streptavidin
BioReagent, suitable for fluorescence
Synonyma:
Streptavidin-Atto 647N
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O této položce
NACRES:
NA.25
UNSPSC Code:
12161900
Technický servis
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BioReagent
Quality Segment
form
powder
manufacturer/tradename
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
UV absorption
λ: 650.0-656.0 nm Amax
suitability
corresponds for gel electrophoresis, suitable for fluorescence
storage temp.
−20°C
General description
Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.
Application
Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.
Features and Benefits
Atto 647N- Streptavidin has the following benefits-
- Highly photostable. Strong absorption.
- Excellent fluorescent quantum yield.
- Highly resistant to ozone degradation.
- Minimal triplet formation.
Analysis Note
dye-to-protein ratio ≥1.5
Skladovací třída
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Sortimentní položky
Barviva Atto jsou řadou fluorescenčních barviv, která splňují kritické potřeby moderních fluorescenčních technologií.
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Související obsah
Spatially resolved two-color diffusion measurements in human skin applied to transdermal liposome penetration
Brewer J, et al.
The Journal of Investigative Dermatology, 133(5), 1260-1268 (2013)
John A Bogovic et al.
PloS one, 15(12), e0236495-e0236495 (2021-01-01)
The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping of individual neurons and neural subtypes. Brain templates are essential for comparative biological studies because they enable
Sub-Diffraction Nano Manipulation Using STED AFM.
Chacko, Jenu V.; et al.
PLoS ONE, 8(6), e66608-e66608 (2013)
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| 94149-1MG | 04061826749715 |