81189

Poly(ethylene glycol)

BioUltra, for molecular biology, 1,000

• Synonyma: Polyethylene glycol, PEG
• Lineární vzorec: H(OCH₂CH₂)_nOH
• Číslo CAS: 25322-68-3
• MDL number: MFCD00081839
• UNSPSC Code: 12352104
• PubChem Substance ID: 24887680
• NACRES: NA.26

Vlastnosti

• grade: for molecular biology
• Quality Level: 100; 200
• product line: BioUltra
• form: solid
• mol wt: M_r 950-1050
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 5.5-7.0 (25 °C, 50 mg/mL in H₂O)
• solubility: H₂O: 50 mg/mL at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤200 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 50 mg/mL in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.10; λ: 280 nm A_max: ≤0.05
• SMILES string: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• InChI key: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Popis

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Application

Poly(ethylene glycol) has been used to develop a method for efficient transformation of Brevibacterium lactofermentum protoplasts with plasmid DNA. It has been used as a fusion agent to fuse spleen cells with the myeloma cell line SP2/0.

Other Notes

PEG precipitation of DNA fragments

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)