50983

Guanidine thiocyanate solution

BioUltra, Molecular Biology, ~6 M in H₂O

• Synonyma: Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate
• Lineární vzorec: NH₂C(=NH)NH₂ · HSCN
• Číslo CAS: 593-84-0
• Molekulová hmotnost: 118.16
• UNSPSC Code: 12352107
• NACRES: NA.25
• PubChem Substance ID: 57651292
• MDL number: MFCD00013027
• Beilstein/REAXYS Number: 3563461

Vlastnosti

• grade: Molecular Biology
• Quality Segment: 200
• product line: BioUltra
• form: liquid
• storage condition: (Tightly closed.; Do not store near acidsTightly closed.; Do not store near acids)
• concentration: ~6 M in H₂O
• technique(s): RNA extraction: suitable
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• color: colorless
• refractive index: n20/D 1.474-1.483
• pH: 4.5-7.0 (25 °C)
• solubility: water: soluble
• anion traces: chloride (Cl^-): ≤500 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤500 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
• absorption: cut-off at 280 nm
• suitability: suitable for molecular biology
• SMILES string: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Popis

General description

Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes. It dissolves proteins by disturbing their secondary structures. Guanidine thiocyanate also separates nucleoproteins from nucleic acids and aids the digestion of the proteins with proteases. It improves the activity of proteinase K thus making it the protease of choice.

Application

Guanidine thiocyanate solution has been used to extract:

• plasma RNA from human immunodeficiency virus (HIV)-infected and control patients
• total RNA from collected tissues (shoots, flowers, fruits, and seeds)
• in reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA from teratocarcinoma cell line (Tera-1)

Disclaimer

possibly crystallized product can be dissolved again by slightly heating to 40-50 °C.

Bezpečnostní informace

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302 + H312 + H332,H314,H412
• pcodes: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• supp_hazards: EUH032
• Skladovací třída: 8A - Combustible corrosive hazardous materials
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter