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38185

Quantification kit for oxidized and reduced glutathione

200 tests

Synonyma:

γ-L-glutamyl-L-cysteinylglycine kit, Quantification kit

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Velikost baleníSkladová položkaDostupnostCena
1 kit
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29 500,00 Kč

O této položce

NACRES:
NA.32
UNSPSC Code:
12352108

29 500,00 Kč


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Quality Level

quality

200 tests

storage temp.

2-8°C

General description

Glutathione Quantification Kit is designed to detect oxidized and reduced Glutathione. Glutathione is usually represented in a reduced form called GSH; however, GSH is converted into its oxidized state (GSSG) upon oxidative stress. The ratio between GSH and GSSG is considered an index for oxidative stress. Glutathione, also known as γ-L-glutamyl-L-cysteinylglycine, is a tripeptide compound that is involved in anti-oxidation and drug metabolism. It acts as a substrate for enzymes like glutathione peroxidase, glutathione S-transferase, and thiol transferase.

Application

Glutathione Quantification Kit is used to detect the total amount of glutathione as an index for oxidative stress or disease risk.

Features and Benefits

The detection ranges of total glutathione and GSSG using in this kit are from 0.5 μmol/l to 50 μmol/l and from 0.5 μmol/l to 25 μmol/l, respectively.

Analysis Note

Glutathione Quantification Kit contains a GSH masking reagent. This enables GSSG detection in the sample by measuring the absorption (λmax = 412 nm) obtained from a colorimetric reaction of DTNB (5,5’-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system. The total quantity of GSH can be measured by subtracting the amount of GSSG from the total amount of glutathione.

Other Notes

Glutathione Quantification Kit contains:
  • Enzyme Solution (50µl X 1)
  • Buffer Solution (60ml X 1)
  • Standard GSH (X 1)
  • Masking Reagent (20µl X 1)
  • Co-enzyme (X 2)
  • Substrate (DTNB) (X4)
  • Standard GSSG (X 1)
not available in Korea, Singapore, Taiwan and Japan

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Tato položka
CGP1GRSAG4251
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

300

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

quality

200 tests

quality

-

quality

-

quality

-


pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - Skin Sens. 1

Skladovací třída

3 - Flammable liquids

wgk

WGK 3



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Související obsah


Anna Maria Czarnecka et al.
Nutrients, 12(7) (2020-07-28)
Acute liver failure (ALF) impairs cerebral function and induces hepatic encephalopathy (HE) due to the accumulation of neurotoxic and neuroactive substances in the brain. Cerebral oxidative stress (OS), under control of the glutathione-based defense system, contributes to the HE pathogenesis.
Bruna Felício Milazzotto Maldonado Porchia et al.
International journal of biological sciences, 18(1), 15-29 (2022-01-04)
The active immunotherapy concept relies on the use of vaccines that are capable of inducing antitumor immunity, reversion of the suppressive immunological environment, and long-term memory responses. Previously, antitumor vaccines based on a recombinant plasmid (pgDE7h) or a purified protein
Geng Tian et al.
Antioxidants & redox signaling, 20(16), 2606-2620 (2013-10-16)
The present study was conducted to define the relationship between the anti-aging effect of ubiquinol-10 supplementation and mitochondrial activation in senescence-accelerated mouse prone 1 (SAMP1) mice. Here, we report that dietary supplementation with ubiquinol-10 prevents age-related decreases in the expression



Globální číslo obchodní položky

Skladová položkaGTIN
38185-1KT04061831961652

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