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48900

Giemsa Stain

Modified Solution

Synonyma:

Azure eosin methylene blue, Giemsa solution

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Vám/Skladová položkaDostupnostCena
100 mL
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1 200,00 Kč
500 mL
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2 460,00 Kč
1 L
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4 300,00 Kč

O této položce

Číslo CAS:
UNSPSC Code:
41116121
PubChem Substance ID:
NACRES:
NA.25
EC Number:
200-659-6
MDL number:

1 200,00 Kč


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Název produktu

Giemsa Stain, Modified Solution, (for the staining (of cellular blood components and blood parasites))

type

(for research use only)

Quality Segment

shelf life

limited shelf life

technique(s)

microbe id | staining: suitable

suitability

Chlamydia spp., Histoplasma spp., Plasmodium spp., Trichomonas spp., bacteria, protozoa, spirochetes

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

Application

Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.

Biochem/physiol Actions

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Physical form

Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)

Preparation Note

Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry

Disclaimer

store at 20°C

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Tato položka
45253GS500GS1L
suitability

Chlamydia spp., Plasmodium spp., bacteria, spirochetes, Trichomonas spp., Histoplasma spp., protozoa

suitability

-

suitability

-

suitability

-

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

-

shelf life

limited shelf life

shelf life

limited shelf life, expiry date on the label

shelf life

Expiry date on the label.

shelf life

Expiry date on the label.

type

(for research use only)

type

-

type

-

type

-

Quality Level

200

Quality Level

100

Quality Level

500

Quality Level

500


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signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Skladovací třída

3 - Flammable liquids

wgk

WGK 2

flash_point_f

53.6 °F

flash_point_c

12 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

target_organs

Eyes,Central nervous system


Seznamy omezení vyplývajících z předpisů

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67-56-1

CAS No.


Vyberte jednu z posledních verzí:

Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  
  1. Does this product usefull for detection of Malaria parasites by thick gout technique?

    1 answer
    1. Giemsa solution has been used since the early 1900s for the diagnosis of malaria.
      Below is a link to a paper from Benin that mentions the relationship between malaria and gout. Uric acid appears to be increased in both conditions. Product 48900 was introduced long before the malaria thick gout procedure was introduced. Since malaria is not endemic to North America or most parts of Europe, positive control slides for use in quality control testing are not available. Since product 48900 is not tested with blood infected with malarial parasites, it is not known how well the product would work for routine malaria identification or in the malaria thick gout method. It is the customer's responsibility to determine if solution 48900 is suitable for the malaria thick gout technique.
      https://austinpublishinggroup.com/infectious-diseases/fulltext/ajid-v8-id1057.php

      Helpful?

  2. WITH WHICH BUFFER SHOULD I DILUTE GIEMSA SOLUTION

    1 answer
    1. Giemsa solution protocols recommend diluting with either water or a phosphate buffer. Common phosphate buffers are Weise or Sorensen's phosphate buffer. Depending upon personal preference, the pH used can vary from a low pH of 6.0 to a high of 7.2 In North America it is more common to use a Sorensen's phosphate buffer with a pH ranging from 6.8 to 7.2. A suitable catalog number for a pH 7.2 buffer is P3288. In other parts of the world slightly more acidic phosphate buffers are preferred.

      Helpful?

  3. Hi does any lab use this stain for microscopy ( haematology) ? How is it working for you on fresh peripheral blood films ?

    1 answer
    1. Giemsa solution is commonly used for hematology, histology, and parasitology labs. Peripheral blood smears and bone marrow smears are routinely stained in hematology labs. It is always preferred to use fresh peripheral blood smears or bone marrow smears when possible. The colors are more vibrant when the peripheral blood smears are stained fresh.

      Helpful?

  4. Is there a white paper available for buffer selection for Giemsa staining?

    1 answer
    1. Generally, no white papers are available. Giemsa formulations vary in pH, typically ranging between pH 6.4 to 6.9. Quality standards for stained slides differ across institutions and individuals, with varying preferences for pH buffers.

      Helpful?

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