Vybrat velikost
3 430,00 Kč
14 700,00 Kč
27 100,00 Kč
83 700,00 Kč
3 430,00 Kč
O této položce
Přeskočit na
Název produktu
KAPA SYBR® FAST, suitable for qPCR, 2 ×, Universal
usage
sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions
sufficient for 5000 reactions
shelf life
≤12 mo.
feature
dNTPs included
hotstart
packaging
kit of 1 mL (100 x 20 μL rxn; KK4600)
kit of 10 mL (1000 x 20 μL rxn; KK4602)
kit of 5 mL (500 x 20 μL rxn; KK4601)
kit of 50 mL (5000 x 20 μL rxn; KK4618)
manufacturer/tradename
Roche
concentration
2 ×
technique(s)
qPCR: suitable
input
purified DNA
compatibility
for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems® 5700
for use with Applied Biosystems® 7000
for use with Applied Biosystems® 7300
for use with Applied Biosystems® 7500
for use with Applied Biosystems® 7700
for use with Applied Biosystems® 7900HT
for use with Applied Biosystems® ViiA 7
for use with QuantStudio™
for use with StepOne™
for use with StepOnePlus™
detection method
SYBR® Green
storage temp.
−20°C
Quality Level
Hledáte podobné produkty? Navštivte Průvodce porovnáváním produktů
1 of 4
Tato položka | QR0100 | SFABIKB | SFLRAB |
|---|---|---|---|
| technique(s) qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) qPCR: suitable | technique(s) qPCR: suitable |
| usage sufficient for 100 reactions | usage sufficient for 250 reactions | usage sufficient for 100 reactions, sufficient for 1000 reactions, sufficient for 500 reactions, sufficient for 5000 reactions | usage sufficient for 100 reactions, sufficient for 1000 reactions, sufficient for 500 reactions, sufficient for 5000 reactions |
| shelf life ≤12 mo. | shelf life - | shelf life ≤12 mo. | shelf life ≤12 mo. |
| feature dNTPs included | feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart |
| packaging kit of 1 mL (100 x 20 μL rxn; KK4600) | packaging - | packaging kit of 1 mL (100 x 20 μL rxn; KK4603), kit of 10 mL (1000 x 20 μL rxn; KK4605), kit of 5 mL (500 x 20 μL rxn; KK4604), kit of 50 mL (5000 x 20 μL rxn; KK4617) | packaging kit of 1 mL (100 x 20 μL rxn; KK4619), kit of 10 mL (1000 x 20 μL rxn; KK4621), kit of 5 mL (500 x 20 μL rxn; KK4620), kit of 50 mL (5000 x 20 μL rxn; KK4622) |
| manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename Roche | manufacturer/tradename Roche |
Analysis Note
Application
Features and Benefits
- High reaction efficiency between 95 - 105% improves accuracy and reproducibility.
- Unbiased efficiency across a wide range of GC contents and amplicon lengths.
Detect low copy and difficult targets consistently
- Improved processivity results in earlier Cq scores.
- Higher fluorescence detection across varying AT- and GC-rich targets.
- Novel enzyme is resistant to SYBR® inhibition.
Complete real-time PCR runs in just 40 minutes
- 55% shorter run times with fast cycling protocol.
- Maintain high performance when switching from standard to fast protocols.
Quick Notes:
- This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
- The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
- 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
- For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
- Do not exceed 25 μL reaction volumes.
General description
The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.
Other Notes
Preparation Note
The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.
Legal Information
signalword
Warning
hcodes
Hazard Classifications
STOT SE 2
Skladovací třída
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Vyberte jednu z posledních verzí:
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Sortimentní položky
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
Související obsah
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
Obraťte se na technický servis