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11767291910

Roche

TUNEL Label Mix

Name: TUNEL Label Mix
Brand: ROCHE
Price: 5210 CZK

sufficient for 30 tests, pkg of 3 × 550 μL

Synonyma:

transferase dUTP nick end labeling, tunel

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Vám/SKU	Dostupnost	Cena
3 x 550 μL	Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.	5 210,00 Kč

O této položce

UNSPSC Code:

41105500

NACRES:

NA.54

Form:

solution

Solubility:

water: miscible

Storage temp.:

−20°C

5 210,00 Kč

Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.

Technický servis

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Vlastnosti

form

solution

usage

sufficient for 30 tests

packaging

pkg of 3 × 550 μL

manufacturer/tradename

Roche

color

colorless

solubility

water: miscible

storage temp.

−20°C

Popis

General description

The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.

Application

TUNEL Label Mix has been used for the determination of apoptosis using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay and DNA nick-end labeling by the TUNEL method.

The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL Enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.

Preparation Note

Working solution: TUNEL Label is used in combination with the TUNEL Enzyme to prepare the TUNEL reaction mixture.
For one test: Mix 45 μl TUNEL Label with 5 μl TUNEL Enzyme prior to use. For negative control use 50 μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45 μl TUNEL Label with 5 μl TUNEL Enzyme for 1 test) should be prepared just before use, and should not be stored. Keep the TUNEL reaction mixture on ice until use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Tato položka	11767305001	APT110	D4001
Roche 11767291910 TUNEL Label Mix	Roche 11767305001 TUNEL Enzyme	Sigma-Aldrich APT110 Apo-Direct TUNEL Assay Kit	Sigma-Aldrich D4001 2′-Deoxyuridine 5′-triphosphate sodium salt
description sufficient for 30 tests, pkg of 3 × 550 μL	description from calf thymus recombinant in E. coli	description The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.	description ≥95%
manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Chemicon^®, ApoDIRECT	manufacturer/tradename -
form solution	form solution	form -	form powder
solubility water: miscible	solubility -	solubility -	solubility -
storage temp. −20°C	storage temp. −20°C	storage temp. -	storage temp. −20°C
color colorless	color -	color -	color -
packaging pkg of 3 × 550 μL	packaging pkg of 2 × 50 μL	packaging -	packaging -

Piktogramy

GHS08,GHS09

Výstražné slovo

Danger

Kódy nebezpečí

H350i,H411

Bezpečnostní pokyny

P201 - P273 - P280 - P308 + P313 - P391 - P501

Hazard Classifications

Aquatic Chronic 2 - Carc. 1B Inhalation

Skladovací třída

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Co se děje?

WGK 3

Bod vzplanutí (°F)

does not flash

Bod vzplanutí (°C)