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Key Documents

11417991001

Roche

SuRE/Cut Buffer H

solution, pkg of 5 × 1 mL

Synonyma:

SuRE/Cut Buffer for Restriction Enzymes, restriction enzyme buffer

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About This Item

UNSPSC Code:
41105600

form

solution

Quality Level

packaging

pkg of 5 × 1 mL

manufacturer/tradename

Roche

storage temp.

−20°C

General description

The SuRE/Cut Buffer System consists of five optimized incubation buffers, 10x concentrated, for DNA restriction digest. Activity of all restriction enzymes has been determined in each buffer to select 100% activity or to calculate activity in double-digests.


Application

SuRE/Cut Buffer H has been used to treat EcoRI and XhoI restriction enzymes to digest gel-purified reaction products and the pMX-IG vector. It has also been used to dilute the restriction enzyme Xanthomonas badrii I for the digestion of the genomic DNA of the E. coli colonies and the Salmonella Braenderup H2812 (ATCC BAA-664) marker.
The SuRE/Cut Buffer H has been used for digestion of genomic DNA using the restriction enzyme XbaI. It has been used for digestion of PCR products and plasmid using EcoRI and XhoI.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

SuRE/Cut is a trademark of Roche

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available


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Sortimentní položky

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Související obsah

Restriction endonucleases in prokaryotes function primarily to protect against foreign genetic material, notably bacteriophage DNA.

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