10875406001
Roche
N-Acetyl-β-D-Glucosaminidase (NAG)
sufficient for ~50 tests
Synonyma:
NAG
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352204
Doporučené produkty
form
solid
Quality Level
usage
sufficient for ~50 tests
mol wt
105.99 g/mol
manufacturer/tradename
Roche
technique(s)
activity assay: suitable
color
white to light yellow-brown
solubility
water: soluble ~210 g/L at 68 °F (Stop reagent)
soluble ~71 g/L at 32 °F (stop reagent)
water: soluble (Buffer substance, lyophilized substrate)
suitability
suitable for enzyme test
application(s)
life science and biopharma
storage temp.
room temp
Související kategorie
General description
N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.
Application
N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.
Packaging
1 kit containing 3 components
Preparation Note
Working solution: Preparation of solutions:
Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.
Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.
- Buffer solution
Dissolve the contents of bottle 1 with 55 ml double-distilledwater. - Substrate solution
Dissolve the contents of bottle 2 with 55 ml solution I. - Stop reagent
Dissolve the contents of bottle 3 with 110 ml double-distilled water.
Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.
Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.
Storage and Stability
Keep container tightly closed in a dry and well-ventilated place.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Pouze součásti soupravy
Č. produktu
Popis
- Buffer Substance consisting of citric acid and potassium citrate
- Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax
- Stop Reagent consisting of sodium carbonate
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
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