SCT205

BioTracker^™ EP-HMRG Green Protease Live Cell Probe

• Synonyma: EP protease probe, EP-HMRG live cell probe, EP-HMRG probe, protease live cell probe
• UNSPSC Code: 41106514
• NACRES: NB.12

Vlastnosti

• manufacturer/tradename: Millipore
• Quality Level: 100
• technique(s): cell based assay: suitable; cell culture | mammalian: suitable; flow cytometry: suitable
• shipped in: dry ice
• storage temp.: −20°C

Popis

General description

BioTracker^™ EP-HMRG is nearly nonfluorescent at pH 7 or higher. It fluoresces by the reaction with proteases that specifically digest the EP (glutamic acid, proline) peptide sequence. A green fluorescent product is membrane permeable and localizes to the lysosome.To use the probe, warm the vial to room temperature and add 30 μL of DMSO or PBS to one vial to make 1 mM solution.Dilute the stock solution with a neutral buffer or culture medium to 1-2 uM and use the diluted solution immediately.SPECTRAL PROPERTIES:Excitation: 500 nmEmission: 524 nmFor fluorescence microscopy, blue excitation filter cubes for FITC or GFP are suitable.™ EP-HMRG Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening.NOTE: Storage of reconstituted probe is not recommended. For best results, discard unused probe solution and make fresh for each use.

Features and Benefits

Live cell imaging probe that reacts with the proteases that digest the peptide sequence of EP (glutamic acid, proline) to fluoresce green by fluorescence microscopy or flow cytometry.

Target description

Early detection of esophageal squamous cell carcinoma (ESCC) is an important prognosticator, but is difficult to achieve by conventional endoscopy. Since fluorescence-based visualization is considered a promising approach, we aimed to develop an activata

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable