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PTEP24H48

Millipore

Millicell® 24 well hanging cell culture inserts

pore size 8.0 μm, translucent PET membrane, diam. 6.5 mm, Tissue Culture (TC)-treated surface, sterile

Synonyma:

cell culture inserts, plate inserts

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About This Item

UNSPSC Code:
41122107
NACRES:
NB.13

material

polystyrene housing
translucent PET membrane

Quality Level

200

sterility

γ-irradiated
sterile

feature

0.2x106 pores/cm2

packaging

pack of 48 ea (individually blister packaged)

parameter

37 °C max. temp.

technique(s)

cell migration: suitable

H

15.5 mm

diam.

6.5 mm

insert maximum volume

0.4 mL

insert working volume

0.2 mL

size

24 wells

surface area

0.3 cm2

color

translucent membrane

pore size

8.0 μm pore size

binding type

Tissue Culture (TC)-treated surface

General description

Millicell®Hanging Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has an 8.0 μm membrane pore size and fits in most24-well plastic tissue culture plates.

In plastic tissue culture plates, cells can access media only from their apical sides, but in Millicell® tissue culture inserts, cells can access media from both their apical and basolateral sides. As result, cell growth, structure, and function more closely mimic what occurs in vivo.

The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.

Application

  • Angiogenesis
  • Chemotaxis
  • Migration / Invasion
  • Feeder Layers
  • Fluorescent Detection / Immunohistochemistry
  • Tumor Cell Metastasis and Invasion

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

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Sortimentní položky

Cell Migration and Invasion Assay Guidance using Millicell® Cell Culture Inserts

How to set up cell migration assays using cell culture inserts.

Tips and Tricks for Choosing a Plate with Millicell® Hanging Inserts

Discover tips for choosing the best plate to use with Millicell® hanging inserts for your applications, including barrier formation and cell imaging.

Protokoly

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

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